Studies on host specificity of actinophage by Carol Koenekamp Ingalsbe

Cover of: Studies on host specificity of actinophage | Carol Koenekamp Ingalsbe

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Statementby Carol Koenekamp Ingalsbe.
The Physical Object
Pagination43 leaves, bound :
Number of Pages43
ID Numbers
Open LibraryOL14311256M

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Download PDF: Sorry, we are unable to provide the full text but you may find it at the following location(s): (external link). The notable biological feature of morbillivirus is its high level of host specificity.

The cellular receptor for a virus is a major determinant of its host specificity and tissue tropism. SLAM is the principal cellular receptor for morbilliviruses allowing entry and propagation [18,19].

SLAM contains two immunoglobulin-like domains, the V and Cited by: 6. Studies on host specificity of actinophageAuthor: Carol Koenekamp Ingalsbe. 3 strains of actinophage (AP-1, AP-2 and AP-3) have been isolated from compost and leaf mould and their specificity has been tested on 10 Studies on host specificity of actinophage book of Streptomyces.

The AP-1 strain lysed Sir. coelicolor, Sir. griseus, Str. scabies and 4 unnamed species, the AP-3 strain lysed the same species except for Str. coelicolor, and the AP-2 strain was strictly monovalent, lysing only an unnamed : F. Mach. Actinophage can be used for host identification at the genus and the species level.

In general, streptomycetes phage are genus-specific, although some cross-reactivity has also been detected with other genera, including Nocardia, Streptosporangium, and Mycobacterium. To obtain a better understanding of actinophage more fundamental studies are required. This development cycle of VWB and the narrow host specificity.

Furthermore, the attachment site. The development cycle of the temperate actinophage VWB was investigated. Adsorption of most phage particles occurred within 30 min and the adsorption constant was x 10(-8) ml min Concluding remarks.

Host specificity is arguably one of the most important properties of a parasite, because it can determine, among other things, whether a parasite can survive the extinction of a host species, whether a parasite has the potential to invade new habitats such as islands, or whether a parasite can become established and spread following its introduction to a new geographical area.

Host range studies Strains listed in Table 1 were assayed against the phage obtained from isolates E and E, respectively. Serial ten-fold dilutions of each phage were prepared in PYCa broth and samples of each dilution were spot-inoculated onto the surface of PYCa plates spread with ml suspension of the test strains.

of the actinomycete host population and its replacement by phage-resistant mutants or lysogenic strains. On this basis, selection pressure by actinophage on the hosts would be very great. However, this argument pre-supposes that the actinophage has uninhibited contact with the host cells in nature, and laboratory experiments with bacteriophage have.

Host specificity normally interferes with phages with hosts from phylogenetically different genera undergoing frequent recombination, but Jacobs-Sera et al. showed that transfection of Zemlya genomic DNA into electrocompetent M.

smegmatis and plating the transfectants in the presence of S. lividans spores can yield plaques at low frequency. The aim of this study was to investigate long-term survival of host and temperate actinophage popu- lations in soil, and determine the major survival strategy of such a phage.

KC is a derivative of the broad host range actinophage ~bC31 [8] which contains the cloned thiostrepton resistance gene, tsr, from S. azureus. Antibiotiki. May-Jun; [Use of actinophage in the identification and directed study of certain producers o polyene antibiotics]. Two types of actinophages, ϕS and ϕL, were isolated from soil samples by usingStreptomyces scabies, a potato scab pathogen, as indicator strain.

The phages were partially characterized according to their physicochemical properties, plaques and particles morphology, and their host range; this varied from narrow (for ϕS) to wide (for ϕL).

The adsorption rate constants of the ϕS and ϕL were. Strain has been utilized as the host of a cosmid vector and in the site-specific recombination of actinophage R4 (), and the integration mechanism between strain and the R4 phage has.

In vitro characterization of the site-specific recombination system based on actinophage TG1 integrase October MGG Molecular & General Genetics (6)   It is known that invertible DNA determines host specificity of bacteriophage Mu (Van de Putte et al., ) and the inversion of specific DNA causes flagellar phase variation of Salmonella (Silverman et al., ).

Thus, the interconversion of pUCl and pUC13 may involve an inversion of a specific DNA segment. Our studies broaden the repertoire of actinophage-based vectors that may find use during the engineering of recombinant S.

ghanaensis strains. Site-specific recombination is a dominant route for pSET, pSOK and pRT integration into the S. ghanaensis chromosome. book provides an introduction to the basic principles and methods of epidemiology.

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Characterization of temperate actinophage phi C31 isolated from Streptomyces coelicolor A3(2). Lomovskaya ND, Mkrtumian NM, Gostimskaya NL, Danilenko VN. Actinophage phiC31 isolated from Streptomyces coelicolor A3(2), the only strain among actinomycetes for which a genetic map had been constructed, appears to be a typical temperate phage.

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The Actinomycetales are an order of Actinobacteria.A member of the order is often called an actinomycete. The actinomycetes are diverse and contain a variety of subdivisions, as well as yet-unclassified isolates, mainly because some genera are difficult to classify because of a highly niche-dependent example, Nocardia contains several phenotypes first believed to be distinct.

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